Detection of Babesia bovis in cattle by PCR-ELISA.

Serological and molecular surveys of Babesia bovis and Babesia bigemina among native cattle and cattle imported from Thailand in Hue, Vietnam

Serum and DNA from blood samples collected from Vietnamese yellow cattle (n=101) and cattle imported from Thailand (n=54) at a Vietnamese slaughter house were screened for Babesia bovis and Babesia bigemina infections by enzyme-linked immunosorbent assay (ELISA) and PCR. The positive rates determined by ELISA (B. bovis and B. bigemina) or PCR (B. bigemina) in the Vietnamese cattle were signific...

A molecular study on Babesia spp. in cattle and ticks in West-Azerbaijan province, Iran

A total number of 450 blood samples were collected from 45 different randomly selected cattle herds. Light microscopic examination of blood smears revealed Babesia spp. infection in 4.2%, while 8.9% of blood samples were positive using PCR. Upon multiplex-PCR (mPCR), B. bigemina and B. bovis infections were detected in 37/40 (92.5%) and 3/40 (7.5%) samples, respective...

Nested PCR Detection and Phylogenetic Analysis of Babesia bovis and Babesia bigemina in Cattle from Peri-Urban Localities in Gauteng Province, South Africa

Babesia bovis and Babesia bigemina are tick-borne hemoparasites causing babesiosis in cattle worldwide. This study was aimed at providing information about the occurrence and geographical distribution of B. bovis and B. bigemina species in cattle from Gauteng province, South Africa. A total of 268 blood samples collected from apparently healthy animals in 14 different peri-urban localities were...

An outbreak of Babesia bovis infection in cattle and its control

Molecular Detection of Babesia bigemina and Babesia bovis in Crossbred Carrier Cattle Through PCR

Bovine babesiosis and other tick borne diseases are considered responsible for more than 50% losses in the crossbred cattle. Carrier cattle infected with babesiosis are challenge to current diagnostic methods and are difficult to detect because of the low number of parasites in circulation. However, diagnosis of carrier animals in herd is important for preventing outbreaks by transmission throu...